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Preheat the oven to 350 degrees. Line 22 to 24 muffin cups with cupcake liners and set pan aside.
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Combine the flour, sugar, cocoa powder, baking soda, and salt in a large mixing bowl and beat with an electric mixer to combine.
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Add the eggs and beat on low just to combine. Add the coffee, sour cream, vegetable oil, and vanilla and beat to combine. Use a rubber spatula to scrape down the sides and bottom of the bowl, then beat on medium speed until batter is smooth.
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Fill each cupcake liner 2/3 full of batter. Bake 16 to 20 minutes, until the cupcakes spring back when lightly touched with your fingertip. Cool cupcakes in pans on wire racks for 20 minutes, then remove from the tins to cool completely before frosting.